Uses of mass spectrometry in carbon dating

posted by | Leave a comment

Agilent Technologies MS software, Biosystems mass spectrometry systems Daltonics, Inc.mass spectrometry systems Ciphergen Biosystems, Inc. contract research organization Biosciences (an affiliate of Merck KGa A) protein purification products Healthcare MS software, protein purification products Pharma Services contract research organization Elmer Life and Analytical Sciences MS software protein purification products Inc.Mass spectrometry is an important method for the characterization and sequencing of proteins.The two primary methods for ionization of whole proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI).Tandem mass spectrometry is usually employed for added specificity.Standard curves and internal standards are used for quantitation of usually a single pharmaceutical in the samples.Results are displayed as spectra of the relative abundance of detected ions as a function of the mass-to-charge ratio.The atoms or molecules in the sample can be identified by correlating known masses to the identified masses or through a characteristic fragmentation pattern.

When the characteristic pattern of peptides is used for the identification of the protein the method is called peptide mass fingerprinting (PMF), if the identification is performed using the sequence data determined in tandem MS analysis it is called de novo peptide sequencing.The samples represent different time points as a pharmaceutical is administered and then metabolized or cleared from the body.Blank or t=0 samples taken before administration are important in determining background and ensuring data integrity with such complex sample matrices.This approach is referred to as “top-down” strategy of protein analysis.The top-down approach however is largely limited to low-throughput single-protein studies.

Leave a Reply

Black adult dating sites no cc